Biological therapeutics of Pongamia pinnata coated zinc oxide nanoparticles against clinically important pathogenic bacteria, fungi and MCF-7 breast cancer cells.

Research paper by Balasubramanian B Malaikozhundan, Baskaralingam B Vaseeharan, Sekar S Vijayakumar, Karuppiah K Pandiselvi, Rajamohamed R Kalanjiam, Kadarkarai K Murugan, Giovanni G Benelli

Indexed on: 25 Jan '17Published on: 25 Jan '17Published in: Microbial Pathogenesis


The overuse of antimicrobics and drugs has led to the development of resistance in a number of pathogens and parasites, which leads to great concerns for human health and the environment. Furthermore, breast cancer is the second most common cause of cancer death in women. MCF-7 is a widely used epithelial cancer cell line, derived from breast adenocarcinoma for in vitro breast cancer studies because the cell line has retained several ideal characteristics particular to the mammary epithelium. In this scenario, the development of novel and eco-friendly drugs are of timely importance. Green synthesis of nanoparticles are cost effective, environmental friendly and do not involve the use of toxic chemicals or elevate energy inputs. This research focused on the antibreast cancer activity of Pongamia pinnata seed extract-fabricated zinc oxide nanoparticles (Pp-ZnO NPs) on human MCF-7 breast cancer cells and their antibiofilm activity against bacteria and fungi. P. pinnata seed extract-fabricated zinc oxide nanoparticles (Pp-ZnO NPs) were characterized by UV-Vis spectroscopy, X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM) and energy dispersive X-ray spectroscopy (EDAX). Pp-ZnO NPs effectively inhibited the growth of Gram positive Bacillus licheniformis (zone of inhibition: 17.3 mm) at 25 μg ml(-1) than Gram negative Pseudomonas aeruginosa (14.2 mm) and Vibrio parahaemolyticus (12.2 mm). Pp-ZnO NPs also effectively inhibited the biofilm formation of C. albicans at 50 μg ml(-1). Cytotoxicity studies revealed that a single treatment with Pp-ZnO NPs significantly reduced the cell viability of breast cancer MCF-7 cells at doses higher than 50 μg ml(-1). Morphological changes in the Pp-ZnO NPs treated MCF-7 breast cancer cells were observed using confocal laser scanning microscopy (CLSM). This study concludes that the green synthesized Pp-ZnO NPs may be used as an effective antimicrobial and antibreast cancer agents.