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Bacteriophage phi297, a new species of Pseudomonas aeruginosa temperate phages with a mosaic genome: Potential use in phage therapy

Research paper by M. V. Bourkal’tseva, S. V. Krylov, A. M. Kropinski, E. A. Pleteneva, O. V. Shaburova, V. N. Krylov

Indexed on: 16 Jul '11Published on: 16 Jul '11Published in: Russian Journal of Genetics



Abstract

The genome of halo-forming temperate Pseudomonas aeruginosa phage phi297 and lytic activity of its virulent mutant were studied. A mosaic structure was revealed for phi297 genome by its complete sequencing. The phi297 genome was partly homologous to the genomes of phages D3 and F116. High lytic activity was assumed for temperate P. aeruginosa bacteriophage phi297 on the basis of morphological features of negative colonies. Virulent mutant phi297vir, which was capable of lysing the wild-type phage bacteria, was isolated. Lytic activity was compared for phi297 and the phages from commercial mixtures of two manufacturers (facilities of Nizhnii Novgorod and Perm’). Phage phi297 caused lysis of the mutant PAO1 bacteria that were resistant to the phages from commercial preparations, but the lytic activity spectrum of phi297 was narrower that the spectra of the commercial phages. The use of nonreverting virulent mutants of certain temperate bacteriophages was proposed for the treatment of P. aeruginosa infections.