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Bacterial cell motility of Burkholderia gut symbiont is required to colonize the insect gut.

Research paper by Jun Beom JB Lee, Jin Hee JH Byeon, Ho Am HA Jang, Jiyeun Kate JK Kim, Jin Wook JW Yoo, Yoshitomo Y Kikuchi, Bok Luel BL Lee

Indexed on: 01 Sep '15Published on: 01 Sep '15Published in: FEBS Letters



Abstract

We generated a Burkholderia mutant, which is deficient of an N-acetylmuramyl-l-alanine amidase, AmiC, involved in peptidoglycan degradation. When non-motile ΔamiC mutant Burkholderia cells harboring chain form were orally administered to Riptortus insects, ΔamiC mutant cells were unable to establish symbiotic association. But, ΔamiC mutant complemented with amiC gene restored in vivo symbiotic association. ΔamiC mutant cultured in minimal medium restored their motility with single-celled morphology. When ΔamiC mutant cells harboring single-celled morphology were administered to the host insect, this mutant established normal symbiotic association, suggesting that bacterial motility is essential for the successful symbiosis between host insect and Burkholderia symbiont.