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Assessment of the Biological Activity in Human Milk Power Treated with Different Processes for Their Conservation

Research paper by Ariana Rodríguez Arreola, Monique Lacroix, Josué Raymundo Solís Pacheco, Elisa García Morales, José Alfonso Gutiérrez Padilla, Eusebio Angulo Castellanos, Juan Arturo Ragazzo Sánchez, Blanca Rosa Aguilar Uscanga

Indexed on: 03 Jun '18Published on: 17 May '18Published in: Journal of Food and Nutrition Research



Abstract

Human milk is the best food for newborns and infants for its combination of nutrients and biological complexity that allows the healthy grows and development of the infant. These components could be affected by thermic treatments of conservation as heat pasteurization, used in Human Milk Banks. The aim of this study was to evaluate the effects of different treatments applied to human milk in its biological activity (antiradical scavenging and anticancer properties). Human milk was heat pasteurized, also submitted high pressure and after it was spray-dried. Moreover, of UV-C or γ- irradiation was applied on spray dried human milk. HT-29 cell line was used for testing antiproliferative assay. The results of this essay showed that 1.25 mg/mL of human milk treated with high pressures and spray dried can attain CI50. Human milk powder treated with γ-irradiation showed the highest inhibition activity of HT-29 cell line, and the pasteurized and spray dried human milk presents the lowest inhibition activity (p<0.05). The highest percentage of scavenging activity against the superoxide anion was in spray-drying treated with γ-irradiation, showing significantly higher activity against the O2 anion as compared to all other treatments tested (p<0.05), followed by human milk powder treated by UV-C irradiation, high pressure and heat pasteurization. Human milk powder treated by High pressure showed higher antioxidant activity, however, the value was not significantly higher as compared to the other pasteurization treatments evaluated (p> 0.05). Spray-dried human milk treated with high pressure, UV-C and γ- irradiation conserved its biological activity, thus it’s could be possible to apply this technology in Human Milk Banks for conserving it.