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Apolipoprotein A-I Stimulates Cell Proliferation in Bone Marrow Cell Culture.

Research paper by I F IF Usynin, A N AN Dudarev, A Yu AY Gorodetskaya, S M SM Miroshnichenko, T A TA Tkachenko, V I VI Tkachenko

Indexed on: 10 Jan '18Published on: 10 Jan '18Published in: Bulletin of Experimental Biology and Medicine



Abstract

Culturing of bone marrow cells in serum-free RPMI-1640 medium led to a decrease in the rate of DNA biosynthesis. Addition of HDL or their main protein component apolipoprotein A-I to the culture medium dose-dependently increased the rate of [3H]-thymidine incorporation into DNA. The maximum stimulation was achieved at HDL concentration of 80 μg/ml and apolipoprotein A-I concentration of 20 μg/ml. To identify the target-cells of apolipoprotein A-I, we used thymidine analogue 5-ethynyl-2'-deoxyuridine (EdU) that incorporates into cell DNA at the stage of replicative DNA synthesis (S phase) and can be detected by fluorescence microscopy. In bone marrow cell culture, apolipoprotein A-I stimulates the proliferation of monocyte (monoblasts, promonocytes) and granulocyte (myeloblasts, promyelocytes) progenitor cells, as well as bone marrow stromal cells.