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ANNALS EXPRESS: Clinical Significance of GCF sIL-6R and Calprotectin to Evaluate the Periodontal Inflammation.

Research paper by Yukari Y Kajiura, Jung Hwan JH Lew, Takahisa T Ikuta, Yasufumi Y Nishikawa, Jun-Ichi JI Kido, Toshihiko T Nagata, Koji K Naruishi

Indexed on: 05 Nov '16Published on: 05 Nov '16Published in: Annals of clinical biochemistry



Abstract

Periodontitis is a inflammatory disease. The aim of this study was to investigate whether soluble form of interleukin-6 receptor (sIL-6R) and calprotectin levels in gingival crevicular fluid (GCF) are useful bio-marker to evaluate the periodontitis.At first, cross-sectional study was performed. A total of 34 periodontitis patients were enrolled and the GCF samples were collected from healthy and inflamed sites of periodontal pockets in each patient. The relationship between periodontal condition and GCF sIL-6R and calprotectin levels was analyzed statistically. Cut-off values of GCF sIL-6R and calprotectin levels to evaluate periodontitis were determined using a receiver operating characteristic curve. Next, it was examined whether calprotectin induces sIL-6R production in THP-1 macrophages using ELISA method.Both GCF sIL-6R and calprotectin levels in inflamed sites were significantly higher than in healthy sites (P<0.0001). Cut-off values of GCF sIL-6R and calprotectin levels to evaluate the periodontal inflammation were as follows: sIL-6R: 43.5 pg/site; calprotectin: 134.3 ng/site. In in vitro study, calprotectin induced significantly sIL-6R production in THP-1 macrophages (P<0.01).Both GCF sIL-6R and calprotectin levels are significant bio-markers to evaluate the periodontal inflammation.