Analysis of connexin intracellular transport and assembly.

Research paper by J K JK VanSlyke, L S LS Musil

Indexed on: 15 Feb '00Published on: 15 Feb '00Published in: Methods


Central to understanding the establishment and regulation of gap junction-mediated intercellular communication is a detailed knowledge of how these complex structures are assembled. In this article, we describe methods to modulate and/or monitor the transport of connexins from the endoplasmic reticulum to the Golgi complex and from the trans Golgi network to the cell surface. We also present techniques that we have developed to assay assembly of newly synthesized connexin monomers into connexons and gap junctional plaques.