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An integrated, multiparametric flow cytometry chip using "microfluidic drifting" based three-dimensional hydrodynamic focusing.

Research paper by Xiaole X Mao, Ahmad Ahsan AA Nawaz, Sz-Chin Steven SC Lin, Michael Ian MI Lapsley, Yanhui Y Zhao, J Philip JP McCoy, Wafik S WS El-Deiry, Tony Jun TJ Huang

Indexed on: 09 May '12Published on: 09 May '12Published in: Biomicrofluidics



Abstract

In this work, we demonstrate an integrated, single-layer, miniature flow cytometry device that is capable of multi-parametric particle analysis. The device integrates both particle focusing and detection components on-chip, including a "microfluidic drifting" based three-dimensional (3D) hydrodynamic focusing component and a series of optical fibers integrated into the microfluidic architecture to facilitate on-chip detection. With this design, multiple optical signals (i.e., forward scatter, side scatter, and fluorescence) from individual particles can be simultaneously detected. Experimental results indicate that the performance of our flow cytometry chip is comparable to its bulky, expensive desktop counterpart. The integration of on-chip 3D particle focusing with on-chip multi-parametric optical detection in a single-layer, mass-producible microfluidic device presents a major step towards low-cost flow cytometry chips for point-of-care clinical diagnostics.