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An Experimental Design Approach for Optimization of Spectrophotometric Estimation of Mirabegron in Bulk and Pharmaceutical Formulations

Research paper by Roopa Kothathi Papanna, Jayanna Bidarur Krishne Gowda, Padmarajaiah Nagaraja

Indexed on: 07 Sep '18Published on: 05 Sep '18Published in: Journal of Analytical Chemistry



Abstract

Three simple, low-cost, sensitive and diversely applicable UV-Vis spectrophotometric methods have been developed for the estimation of drug Mirabegron. Method A is based on the reaction of Mirabegron with ninhydrin in the presence of sodium molybdate at pH 5.5. Method B is based on the reaction of the drug with 1,2-napthaquinone-4-sulphonate and cetyltrimethyl ammonium bromide in an alkaline medium. Method C is based on a redox reaction of the drug with Folin–Ciocalteu reagent in sodium carbonate medium. Beer’s law was obeyed in the concentration ranges of 2.5–22.5, 5–35, and 5–70 μg/mL for methods A, B, and C. The proposed methods can be applied to drug formulation and recommended for the routine analysis in quality control laboratories.