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An autoradiographic technique for the detection of cystine and peptides containing cystine or its derivatives in keratin digests

Research paper by G. E. Mathison

Indexed on: 01 Dec '65Published on: 01 Dec '65Published in: Mycopathologia



Abstract

A technique is described for the detection of cystine or peptides containing cystine or its derivatives in digests of keratin. The technique involves the preparation of radioactive wool by the intravenous injection of experimental animals with (35S) cystine followed by the growth of keratinolytic fungi upon the labelled wool and the detection of soluble compounds in the wool digests which contain cystine or its derivatives by paper chromatography and autoradiography.