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Affinity capture elution bridging assay: A novel immunoassay format for detection of anti-therapeutic protein antibodies

Research paper by Yan Q. Chen, Thomas G. Pottanat, Quincy L. Carter, Jason S. Troutt, Robert J. Konrad, John H. Sloan

Indexed on: 18 Mar '16Published on: 10 Feb '16Published in: Journal of Immunological Methods



Abstract

Increased emphasis on the development of biologics has placed a significant focus on anti-drug antibody (ADA) detection. To address this need, several immunoassay formats have been described for use in characterizing potential immune responses. Two commonly utilized methods include the affinity capture elution (ACE) and bridging formats. While these approaches have been effective in supporting many clinical initiatives, both possess potential disadvantages. Here, we compare these standard methods to a novel format that addresses these noted drawbacks.

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