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Advances in synthetic dynamic circuits design: using novel synthetic parts to engineer new generations of gene oscillations.

Research paper by Steven William SW Sowa, Grant G Gelderman, Lydia Maria LM Contreras

Indexed on: 08 Sep '15Published on: 08 Sep '15Published in: Current Opinion in Biotechnology



Abstract

As bioengineering applications expand, the need to design and implement circuits that exhibit dynamic properties increases. In particular, schemes that control precise patterns of gene expression as a function of time are essential for balancing multiple metabolic objectives in natural and synthetic systems. Given that modularity has been an important component of dynamic circuits, recent efforts to improve dynamic circuits have focused on replacing old parts for new components that increase the robustness, stability, and tunability. In this review, we show that incorporation of novel components such as regulatory noncoding RNAs (ncRNAs), promoter-transcription factor pairs, and metabolite sensors have allowed traditional dynamic circuits to obtain more robust functionality and improved dynamic properties.