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Advances in phase plate cryo-EM imaging of DNA and nucleosomes.

Research paper by Eugene Y D EY Chua, Sara S Sandin

Indexed on: 25 Mar '17Published on: 25 Mar '17Published in: Nucleus (Austin, Tex.)



Abstract

Contrast in electron cryo-microscopy (cryo-EM) is limited by the weak phase and radiation sensitive nature of biologic samples embedded in vitrified ice. We have recently shown that a new contrast enhancement technique utilizing the Volta phase plate can be combined with single particle analysis to determine the structure of a small chromatin complex, the nucleosome core particle, at near-atomic resolution. Here, we discuss advantages and limitations of the technique in terms of data collection, particle detection, and visualization of individual DNA molecules and higher-order chromatin structure.