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A translational enhancer element on the 3'-proximal end of the Panicum mosaic virus genome.

Research paper by Jeffrey S JS Batten, Benedicte B Desvoyes, Yoshimi Y Yamamura, Karen-Beth G KB Scholthof

Indexed on: 02 May '06Published on: 02 May '06Published in: FEBS Letters



Abstract

Panicum mosaic virus (PMV) is a single-stranded positive-sense RNA virus in the family Tombusviridae. PMV genomic RNA (gRNA) and subgenomic RNA (sgRNA) are not capped or polyadenylated. We have determined that PMV uses a cap-independent mechanism of translation. A 116-nucleotide translational enhancer (TE) region on the 3'-untranslated region of both the gRNA and sgRNA has been identified. The TE is required for efficient translation of viral proteins in vitro. For mutants with a compromised TE, addition of cap analog, or transposition of the cis-active TE to another location, both restored translational competence of the 5'-proximal sgRNA genes in vitro.