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A simple method for determination of the proportion of 2C and 4C cells in a dormant embryo

Research paper by Zia U. Ahmed, Latifa Akhtar

Indexed on: 01 Aug '81Published on: 01 Aug '81Published in: Cellular and Molecular Life Sciences



Abstract

Pea embryos grown in the presence of 2.5 mM hydroxyurea from the beginning of imbibition showed a mitotic peak the height of which was considerably less than that of the control, material. Soon after the mitotic peak, there was a complete cessation of mitosis in the treated material. The extent of suppression of mitosis during the first post-dormancy cell cycle caused by hydroxyurea has been used to obtain an estimate of the relative proportion of 2C and 4C cells in the embryo.