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A simple and modified manometric method for measuring oxygen uptake rate of plant cells in flask cultures

Research paper by Zhi-You Wen, Jian-Jiang Zhong

Indexed on: 01 Jul '95Published on: 01 Jul '95Published in: Biotechnology Techniques



Abstract

A simple and convenient technique was developed based on the principle of Warburg manometric method to measure O2 uptake rate (OUR) and CO2 evolution rate (CER) of suspended cells in a shake flask culture. It was successfully applied to suspension cultures of rice (Oryza sativa) and Panax notoginseng cells, and some important bioprocess parameters, such as OUR, CER, respiratory quotient (RQ), specific OUR (SOUR) and specific CER (SCER), were quantitatively obtained. The measuring system is easy to operate, able to treat many samples simultaneously and is economical.