A potential procedure for obtaining mutants of Escherichia coli that affect plasmid stability

Research paper by V. N. Iyer, I. Messmer

Indexed on: 01 Jun '74Published on: 01 Jun '74Published in: Molecular & general genetics : MGG


A procedure involving the infection of a RecA-F prime strain of Escherichia coli with the bacteriophage M13 followed by selection for the plasmid-associated markers was tested as one that might yield those rare mutants in which plasmid stability is increased. The mutations in two strains isolated in this manner were chromosomal. The rationale of the procedure, its possible extension to other strains and plasmids and its limitation are briefly discussed.