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A novel type of EWS-CHOP fusion gene in myxoid liposarcoma.

Research paper by Yoshito Y Matsui, Takafumi T Ueda, Takahiro T Kubo, Tadashi T Hasegawa, Yasuhiko Y Tomita, Mina M Okamoto, Akira A Myoui, Shigeki S Kakunaga, Natsuo N Yasui, Hideki H Yoshikawa

Indexed on: 04 Aug '06Published on: 04 Aug '06Published in: Biochemical and Biophysical Research Communications



Abstract

The cytogenetic hallmark of myxoid type and round cell type liposarcoma consists of reciprocal translocation of t(12;16)(q13;p11) and t(12;22)(q13;q12), which results in fusion of TLS/FUS and CHOP, and EWS and CHOP, respectively. Nine structural variations of the TLS/FUS-CHOP chimeric transcript have been reported, however, only two types of EWS-CHOP have been described. We describe here a case of myxoid liposarcoma containing a novel EWS-CHOP chimeric transcript and identified the breakpoint occurring in intron 13 of EWS. Reverse transcription-polymerase chain reaction and direct sequence showed that exon 13 of EWS was in-frame fused to exon 2 of CHOP. Genomic analysis revealed that the breaks were located in intron 13 of EWS and intron 1 of CHOP.