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A new method of DNA preparation for pulsed-field gel electrophoresis analysis.

Research paper by Janusz J Fiett, Krzysztof K Kucharczyk, Marek M Gniadkowski

Indexed on: 19 Oct '04Published on: 19 Oct '04Published in: Journal of Microbiological Methods



Abstract

We have developed a new, labour-saving method of preparation, handling and treatment of DNA-containing agarose plugs for pulsed-field gel electrophoresis (PFGE). A plastic mould in which plugs are formed and supported during DNA purification and digestion was designed and successfully tested in a prototype device.