A molecular fluorescent dye for specific staining and imaging of RNA in live cells: a novel ligand integration from classical thiazole orange and styryl compounds.

Research paper by Yu-Jing YJ Lu, Qiang Q Deng, Dong-Ping DP Hu, Zheng-Ya ZY Wang, Bao-Hua BH Huang, Zhi-Yun ZY Du, Yan-Xiong YX Fang, Wing-Leung WL Wong, Kun K Zhang, Cheuk-Fai CF Chow

Indexed on: 04 Sep '15Published on: 04 Sep '15Published in: Chemical Communications


A new RNA-selective fluorescent dye integrated with a thiazole orange and a p-(methylthio)styryl moiety shows better nucleolus RNA staining and imaging performance in live cells than the commercial stains. It also exhibits excellent photostability, cell tolerance, and counterstain compatibility with 4',6-diamidino-2-phenylindole for specific RNA-DNA colocalization in bioassays.