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A method for the 32P labeling of peptides or peptide nucleic acid oligomers.

Research paper by I A IA Kozlov, P E PE Nielsen, L E LE Orgel

Indexed on: 21 Jul '98Published on: 21 Jul '98Published in: Bioconjugate Chemistry



Abstract

A novel approach to the radioactive labeling of peptides and PNA oligomers is described. It is based on the conjugation of a deoxynucleoside 3'-phosphate with the terminal amine of the substrate, followed by phosphorylation of the 5'-hydroxyl group of the nucleotide using T4 polynucleotide kinase and [gamma-32P]ATP.