A group-specific sequence-based typing approach for HLA-DQA1.

Research paper by A J AJ Lemin, J J Johnson, C C Darke

Indexed on: 30 Dec '14Published on: 30 Dec '14Published in: International Journal of Immunogenetics


An HLA-DQA1 sequence-based typing method reliant upon group-specific amplification to achieve an unambiguous second-field DQA1 typing assignment is presented. Method validation, using 51 reference DNA samples covering 21 different DQA1 alleles, showed 100% concordance with the reference types. This typing strategy has several important uses including identifying DQA1 mismatches in kidney donor/recipient pairs to inform patient DQ antibody assignments.