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A general protein purification and immobilization method on controlled porosity glass: biocatalytic applications.

Research paper by K K Engelmark Cassimjee, M M Kadow, Y Y Wikmark, M M Svedendahl Humble, M L ML Rothstein, D M DM Rothstein, J-E JE Bäckvall

Indexed on: 06 Jul '14Published on: 06 Jul '14Published in: Chemical Communications



Abstract

A general combined purification and immobilization method to facilitate biocatalytic process development is presented. The support material, EziG™, is based on controlled porosity glass (CPG) or polymer-coated versions thereof (HybCPG) and binds protein affinity tags. Biocatalytic reactions in aqueous and organic media with seven enzymes of biocatalytic interest are shown.