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2D-Electrophoresis and Immunoblotting in Food Allergy.

Research paper by Galina G Grishina, Luda L Bardina, Alexander A Grishin

Indexed on: 21 Mar '17Published on: 21 Mar '17Published in: Methods in molecular biology (Clifton, N.J.)



Abstract

Two-dimensional polyacrylamide gel electrophoresis (2DE) and Western immunoblotting have proven to be invaluable and fundamental techniques for comprehensive characterization of food allergens. Here, we describe and discuss detailed protocols used in our studies on identification of allergenic proteins in shrimp, sesame, hazelnut, and pistachio.