2,5-Diketo- D-gluconate production by a mixed culture of two newly-isolated strains: Flavimonas oryzihabitans and Pseudomonas cepacia

Research paper by Pavol Sulo, Daniela Hudecová, Antónia Properová, Ivan Bašnák, Ivo Sedláček

Indexed on: 01 May '01Published on: 01 May '01Published in: Biotechnology Letters


A mixed culture of two Gram-negative bacteria isolated from soil converted 50 g d-glucose l−1 to 2,5-diketo-d-gluconate (2,5 DKG) in 92% yield within 150 h. The first strain, producing 2-keto-d-gluconate (2 KDG) from d-glucose via d-gluconate (DG), was classified as Flavimonas oryzihabitans. The second strain, that converts 2 KDG to 2,5 DKG, was identified as Pseudomonas cepacia. This approach presents a new possibility to produce ascorbic acid by microbial transformation, including the use of other, more convenient substrates.